Estrogens, Total

Test Details

Use

Evaluate for ovarian estrogen producing tumor in the premenarcheal and postmenopausal female; evaluate estrogen excess in males. Estrogen analysis may be helpful in establishing time of ovulation and optimal time for conception. Serial samples must be collected over several days to evaluate baseline and peak total estrogen levels.

Methodology

Enzyme-linked immunoassay (ELISA)

References

Ashby CD, Danzer HC, Swerdloff RS, et al. Estrogen radioimmunoassay suitable for the monitoring of ovulation induction. Clin Chem. 1980; 26(8):1143-1146. 7389084
Marshall JR. Induction of ovulation. Clin Obstet Gynecol. 1978; 21(1):147-162 (review). 343954
Taymor ML, Yussman MA, Gminski D, et al. Estrogen monitoring in ovulation induction. Fertil Steril. 1970; 21(11):759-762.5474219

Specimen Requirements

Information on collection, storage, and volume

Specimen

Serum

Volume

0.8 mL

Minimum Volume

0.5 mL (Note: This volume does not allow for repeat testing.)

Container

Red-top tube or gel-barrier tube

Storage Instructions

Room temperature

Causes for Rejection

Gross hemolysis; gross lipemia; icteric specimen

Collection

If tube other than a gel-barrier tube is used, transfer separated serum to a plastic transport tube.