ABO Grouping and Rho(D) Typing

CPT 86900; 86901
Synonyms
  • Blood Grouping and Rh Typing
  • Blood Type
  • Group and Type
  • Type and Rh

Test Details

Use

Determination of ABO blood group and Rh type for transfusion candidates. Group and typing of expectant mothers and newborns may indicate potential for ABO hemolytic disease of the fetus/newborn. Rh typing is used to determine Rh immune globulin candidacy for prenatal and postpartum patients.

Special Instructions

If a CBC and/or an antibody screening test is ordered with this test, please submit a separate 4 mL specimen for each test.

Test Includes

ABO blood grouping and Rh typing

Limitations

Abnormal plasma proteins, cold autoagglutinins, positive direct antiglobulin test, and, in some cases, bacteremia may interfere with test interpretation. Variable testing results may be observed with partial antigens, which are rare variants of the D antigen.

Methodology

Hemagglutination

References

Fung MK, Grossman JB, Hillyer CD, Westoff CM, eds. AABB Technical Manual. 18th ed. Bethesda, Md: American Association of Blood Banks;2014.

Specimen Requirements

Information on collection, storage, and volume

Specimen

Whole blood

Volume

4 mL

Minimum Volume

1 mL

Container

Lavender-top (EDTA) tube

Storage Instructions

Specimens should be tested as soon as possible. Samples that cannot be tested immediately should be stored at 2°C to 8°C. Do not freeze red cells.

Causes for Rejection

Gross hemolysis; bacterial or other contamination