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Turnaround Time
1 - 4 days
Use
Identification of red cell antibodies and titration of those antibodies which may be clinically significant during pregnancy or transfusion. This test is automatically performed whenever the antibody screening test is positive.
Special Instructions
This test should only be ordered if the patient has a previous history of a positive antibody screening test.
Test Includes
Antiglobulin titer; red cell antibody identification
Limitations
Specimens drawn within six months after administration of Rh immune globulin may contain passively administered anti-D. If the mother is Rh-negative, her specimen should be collected before Rh immune globulin is administered. Extremely low-titered antibodies may be too weak to identify.
Methodology
Antiglobulin test
References
Fung MK, Grossman JB, Hillyer CD, Westoff CM, eds. AABB Technical Manual. 18th ed. Bethesda, Md: American Association of Blood Banks;2014.
Specimen Requirements
Information on collection, storage, and volume
Specimen
Whole blood
Volume
8 mL whole blood
Minimum Volume
4 mL whole blood
Container
Lavender-top (EDTA) tube. Do not use a gel-barrier tube.
Storage Instructions
Specimens should be tested as soon as possible or within 72 hours of collection. Samples that cannot be tested immediately should be stored at 2°C to 8°C. Do not freeze.
Causes for Rejection
Gross hemolysis; bacterial or other contamination