Cytogenetics Amniotic Fluid Chromosome Analysis

CPT 88235; 88267; 88280; 88285
Synonyms Karyotype

Test Details

Turnaround Time

9 - 12 days Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.


Determine fetal karyotype.

Special Instructions

Please call our Client Services Department prior to sending first specimen. If additional testing such as DNA is indicated, call Client Services for further specimen requirements. If prior NIPT or maternal serum screening studies have been performed, include copy of report.

Test Includes

15-20 mitotic spreads counted from separate in situ colonies and 2 banded karyotypes prepared

Specimen Requirements

Information on collection, storage, and volume


Amniotic fluid


20-30 mL (15-20 mL for early amniocentesis)


Two 15 mL sterile orange-top tubes

Storage Instructions

Maintain specimen at room temperature. Do not freeze.

Causes for Rejection

Frozen specimen; hemolysis; quantity not sufficient for analysis; improper container; unlabeled or mislabeled specimen)


Discard first 2 mL; then 15-25 mL amniotic fluid (less if early amniocentesis)