Hemoglobinopathy Fractionation Cascade

Test Details

Use

Diagnose thalassemias and hemoglobin variants

Special Instructions

State the patient's age, ethnic background, MCV, transfusion history, and any other known hematological data on the test request form.

Test Includes

Capillary electrophoresis (CE) is performed initially. If an abnormal hemoglobin is detected, appropriate testing will be performed at an additional charge. Depending on findings, one or more reflexive tests may be required in order to provide a clinical interpretation. These tests may include high-pressure liquid chromatography (HPLC) and/or hemoglobin solubility testing.

Methodology

Capillary electrophoresis (CE); high-pressure liquid chromatography (HPLC); Hgb Solubility: Sodium hydrosulfite reduction

Specimen Requirements

Information on collection, storage, and volume

Volume

1.5 mL

Minimum Volume

0.8 mL (Note: This volume does not allow for repeat testing.)

Container

Lavender-top (EDTA) tube

Storage Instructions

Refrigerate at 2°C to 8°C.

Causes for Rejection

Clotted specimen; aged, improperly stored blood samples

Collection

To avoid delays in turnaround time, please submit a separate lavender-top tube for each test requiring a lavender-top.