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Turnaround Time
2 - 3 days
Use
Enzyme activity test for use in the detection of vaginal fluid specimens for sialidase activity, an enzyme produced by bacteria associated with bacterial vaginitis, including Bacteroides spp, Prevotella spp, and Mobiluncus spp and some G vaginalis. In a recent study, Bradshaw et al demonstrated the test to be 88% sensitive and 95% specific.
Limitations
Collecting specimens from the cervix should be avoided because (a) it might increase risk to OB patients, and (b) cervical sialidase activity is usually higher than vaginal sialidase activity. Do not collect specimens from patients who have (a) used a vaginal cream or ointment product, (b) douched, or (c) used spermicides, vaginal lubricants, or feminine sprays within 72 hours of testing. If insufficient sample is collected or collected from a patient undergoing antimicrobial therapy, the test may give a false-negative result.
Methodology
Chromogenic enzyme activity test
Related Documents
For more information, please view the literature below.
References
Bradshaw CS, Morton AN, Garland SM, Horvath LV, Kuzevska I, Fairley CK. Evaluation of a point-of-care test, BVBlue, and clinical laboratory criteria for diagnosis of bacterial vaginosis. J Clin Microbiol. 2005 Mar; 43(3):1304-1308. 15750100
Specimen Requirements
Information on collection, storage, and volume
Specimen
Vaginal fluid
Volume
Swab
Container
Copan double swab in Amies liquid medium
Storage Instructions
Maintain specimen at room temperature for up to 48 hours.
Causes for Rejection
Specimen collected or transported other than previously described
Collection
Using transport swabs provided, obtain a sample by contacting the lower one-third of the vaginal wall, rotating 10 to 20 seconds to absorb fluid. Immediately place the swabs into the transport tube.