Maternal Serum Alpha-Fetoprotein (MSAFP)

CPT 82105
Synonyms AFP, Maternal Serum; Alpha-Fetoprotein (AFP), Maternal Serum for Open Spina Bifida; Maternal Serum a-Fetoprotein; msAFP;

Test Details

Turnaround Time

2 - 5 days Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.


Risk assessment for fetal open neural tube defects. At 15w-23w6d (optimal 16w-18w6d)


Immunochemiluminometric assay (ICMA)

Specimen Requirements

Information on collection, storage, and volume


Whole blood or serum (unhemolyzed)


Serum: 2-3 mL Whole Blood: 7 mL


Serum: AFP tube Whole Blood: Serum separator tube (SST) or red-top tube

Storage Instructions


Causes for Rejection

Gross hemolysis; gross lipemia; quantity not sufficient for analysis


Whole Blood: allow to clot; centrifuge (15 minutes at 1,500 rpm) to prevent hemolysis.