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Synonyms
AFP, Maternal Serum; Alpha-Fetoprotein (AFP), Maternal Serum for Open Spina Bifida; Maternal Serum a-Fetoprotein; msAFP;
Test Details
Turnaround Time
2 - 5 days Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.
Use
Risk assessment for fetal open neural tube defects. At 15w-23w6d (optimal 16w-18w6d)
Methodology
Immunochemiluminometric assay (ICMA)
Specimen Requirements
Information on collection, storage, and volume
Specimen
Whole blood or serum (unhemolyzed)
Volume
Serum: 2-3 mL Whole Blood: 7 mL
Container
Serum: AFP tube Whole Blood: Serum separator tube (SST) or red-top tube
Storage Instructions
Room temperature.
Causes for Rejection
Gross hemolysis; gross lipemia; quantity not sufficient for analysis
Collection
Whole Blood: allow to clot; centrifuge (15 minutes at 1,500 rpm) to prevent hemolysis.