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Afp4® Screening - Quadruple Marker Test (AFP, hCG, uE3, Inhibin A)

CPT: 82105; 82677; 84702; 86336


AFP Tetra; Alpha-Fetoprotein (AFP) Tetra Profile;

Test Includes

α-Fetoprotein, serum; dimeric inhibin A (DIA); human chorionic gonadotropin (hCG); unconjugated estriol (uE3)

Special Instructions

Please Note: Ultrasound BPD (biparietal diameter) dating of pregnancy is preferred for optimal accuracy of risk calculations.

Expected Turnaround Time

2 - 5 days Turnaround time is defined as the usual number of days from the date of pickup of a specimen for testing to when the result is released to the ordering provider. In some cases, additional time should be allowed for additional confirmatory or additional reflex tests. Testing schedules may vary.

Specimen Requirements


Whole blood; serum


Serum: 2-3 mL (unhemolyzed). Whole Blood: 7 mL in SST or 10 ml in red-top tube; allow to clot; centrifuge (15 minutes at 1,000 g) to prevent hemolysis.


Serum: AFP tube Whole Blood: Serum separator tube (SST) or red-top tube.


Avoid hemolysis. Send complete specimen in the original tube. Do not pour off.

Storage Instructions

Maintain at room temperature

Causes for Rejection

Gross hemolysis; gross lipemia; quantity not sufficient for analysis

Test Details


Screening test for open neural tube defects (detects 80% open spina bifida, 90% anencephaly), Down syndrome (detects 75% to 80%), and trisomy 18 (detects 73%)


Immunochemiluminometric assay (ICMA)

Pregnancy Week

14-21 Weeks