Semen Analysis, Basic

Test Details

Use

Diagnose male factor infertility, including oligozoöspermia, azoöspermia, asthenozoöspermia, and teratozoöspermia

Special Instructions

This test is not available at all locations. Contact the laboratory prior to specimen collection. This procedure must be scheduled with the laboratory performing the test. The patient should have between two and seven days of sexual abstinence before producing the specimen. This test requires a fresh specimen; therefore, this procedure is available only at sperm testing facilities.

Methodology

Manual macroscopic and microscopic examination of semen

References

World Health Organization. WHO Laboratory Manual for the Examination and Processing of Human Semen. 5th ed. Geneva, Switzerland: WHO; 2010.

Specimen Requirements

Information on collection, storage, and volume

Specimen

Semen

Volume

Entire ejaculate

Minimum Volume

Approximately 0.5 mL

Container

Sterile screw-cap container

Storage Instructions

Testing must begin within 60 minutes of specimen production. In the interim, the specimen should be kept at room temperature, but it is recommended that it be kept close to the body (inside a shirt or coat) to avoid temperature extremes during transport.

Causes for Rejection

Specimens older than one hour; insufficient sperm (may prevent adequate estimate of percent normal morphology)

Collection

See Patient Instructions for Semen Collection.